Respiratory System Infection Pathogen Targeted Next-generation Sequencing (tNGS) is an accurate detection method that combines the advantages of ultra-high multiplex target amplification and high-throughput sequencing technology. By extracting and enriching nucleic acids from sputum and bronchoalveolar lavage fluid, using the sequencing platform to sequence the pathogen's genes, and comparing and analyzing sequence information against a database, this kit can identify 183 types of respiratory system infection pathogens such as DNA/RNA viruses, bacteria, fungi, and mycoplasma, as well as 34 types of drug-resistant genes/mutation sites, assisting in precise clinical diagnosis.